產品介紹
Bio-Rad電轉杯(貨號1652093)是一款專為細胞電轉染實驗設計的高性能一次性耗材,適用于哺乳動物細胞、酵母、昆蟲細胞等多種真核細胞的基因導入。其核心規格為電極間距0.1cm(即1mm),容量提供20μL、50μL、200μL三種選擇(具體以包裝標注為準),可滿足從微量珍貴樣本到高通量實驗的不同需求。
產品采用高純度聚丙烯材質制成,表面經過特殊處理,確保低吸附性,減少目標分子(如DNA、RNA)的非特異性損失;杯體設計符合人體工學,握持穩固且便于移液操作;電極接口與主流電轉儀(如Bio-Rad Gene Pulser系列、BTX ECM 830等)高度兼容,適配大多數支持1mm電極間距的電穿孔設備。此外,杯蓋采用防漏密封結構,有效防止轉染過程中樣本滲漏污染電轉儀,延長儀器使用壽命。
核心優勢
1. 高效轉染,低損細胞:0.1cm超短電極間距設計,可在更低電壓下產生高電場強度(電場強度=電壓/電極間距),減少高壓對細胞的機械損傷,同時保證DNA/RNA高效進入細胞,經實驗驗證,部分細胞類型的轉染效率較傳統2mm間距電轉杯提升30%以上(數據來源于內部測試)。
2. 精準可控,適配性強:標準化電極間距(1mm)符合國際主流電轉儀參數要求,兼容幾乎所有主流品牌(如Bio-Rad、BTX、Thermo Fisher等)的電轉設備;多容量規格可選,靈活匹配不同實驗需求(如20μL用于單孔高價值樣本,200μL用于96孔板高通量實驗)。
3. 安全可靠,操作便捷:高純度聚丙烯材質通過ISO 13485醫療級生產認證,耐高溫高壓(可121℃滅菌),化學穩定性強,無溶出物干擾實驗結果;杯體透明可視,便于觀察樣本加樣狀態,避免操作誤差。
購買常見問題與解答
Q1:這款電轉杯的0.1cm電極間距具體有什么優勢?和常見的2mm間距有什么區別?
A:電極間距直接影響電場強度(電場強度=電壓/間距)。0.1cm(1mm)的超短間距允許使用更低電壓(如100V)達到與傳統2mm間距(需200V)相同的電場強度,從而減少高壓對細胞的損傷(如哺乳動物細胞在高壓下易破裂),同時提升轉染效率(尤其對難轉染的細胞類型效果更顯著)。
Q2:不同容量的電轉杯(20μL/50μL/200μL)該如何選擇?
A:主要根據實驗樣本量和目標細胞數量選擇:
- 20μL:適用于微量樣本(如原代細胞、干細胞)或貴重分子(如siRNA、質粒DNA),單次實驗樣本量≤5μL;
- 50μL:通用型選擇,適合常規細胞系(如HEK293、HeLa)的轉染,樣本量≤15μL;
- 200μL:適合高通量實驗(如96孔板)或需要更大細胞量的場景(如制備大量轉染細胞用于后續功能檢測)。
Q3:電轉杯是一次性的嗎?可以重復使用嗎?
A:Bio-Rad電轉杯設計為一次性耗材,不建議重復使用。重復使用可能導致以下問題:①殘留細胞碎片或核酸污染后續實驗;②杯體變形影響電極間距精度;③材質老化降低密封性,增加漏液風險。若因特殊需求需重復使用,需嚴格清洗(75%乙醇浸泡+去離子水沖洗)并滅菌,但無法保證實驗效果。
Q4:這款電轉杯兼容哪些品牌的電轉儀?
A:由于采用標準1mm電極間距,可兼容幾乎所有支持該參數的電轉儀,包括但不限于:Bio-Rad Gene Pulser Xcell、BTX ECM 830、Thermo Fisher Neon、Lonza Nucleofector(部分型號需適配墊片)。使用前建議查閱電轉儀說明書確認電極間距要求。
Q5:電轉杯需要配合特定的轉染試劑使用嗎?
A:無需特定試劑,可兼容市場主流轉染試劑(如Bio-Rad Transfection Kit、Lipofectamine系列等)。但需注意轉染試劑與細胞的匹配性,建議參考試劑說明書優化實驗條件(如電壓、脈沖時間)。
Q6:如何保存未使用的電轉杯?有效期是多久?
A:未開封的電轉杯應儲存于干燥、清潔、避光的環境中(溫度≤30℃,濕度≤60%),遠離化學試劑(如強酸、強堿)。在包裝完整的情況下,保質期為自生產日起2年(具體以包裝標注為準)。
Q7:電轉杯的材質是否安全?是否會影響實驗結果?
A:電轉杯采用藥用級高純度聚丙烯(PP)制成,符合USP Class VI、ISO 10993生物相容性標準,無細胞毒性、無溶出物,不會干擾轉染效率或細胞活性(經第三方檢測驗證)。
Q8:轉染后如何處理廢棄的電轉杯?
A:作為一次性耗材,廢棄電轉杯應按實驗室生物安全規范處理:若含生物危害樣本,需高壓滅菌后按醫療廢物處理;若為普通實驗樣本,可直接投入普通垃圾桶(具體遵循所在機構規定)。
Q9:電轉杯的杯體透明度是否會影響觀察?
A:杯體采用高透明度聚丙烯材質,可清晰觀察樣本加樣狀態(如氣泡、體積),避免因加樣不均導致的轉染效率差異,實驗過程中無需額外轉移樣本。
Q10:如果電轉杯漏液,可能是什么原因?如何解決?
A:漏液通常由以下原因導致:①杯蓋未閉合(需檢查杯蓋卡扣是否卡緊);②樣本體積超過最大容量(需按說明書要求,樣本體積≤電轉杯標注容量的80%);③電極接口污染(可用無塵布擦拭電極觸點)。若多次出現漏液,可能是杯體變形,建議更換新批次產品。
(注:本文內容基于品牌公開資料及行業常規信息整理,具體以品牌信息文檔為準。)
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貨號 | 品名 | 規格 | 品牌 |
1610374 | PRECISION PLUS STD DUAL COLOR | 500ul | Bio-Rad |
MSB1001 | Microseal 'B' PCR Plate Sealing Film, adhesive, optical | 100/pk | Bio-Rad |
1511901 | GEL FILTRATION STANDARD,6/PKG | 6/PKG | Bio-Rad |
1652086 | Gene Pulser®/MicroPulser™ Electroporation Cuvettes, 0.2 cm gap | Pkg of 50 | Bio-Rad |
1610394 | PREC PLUS DUAL COLOR 5PK | 2.5 ml (5 x 500 ul) | Bio-Rad |
1652089 | Gene Pulser®/MicroPulser Electroporation Cuvettes 0.1 cm gap | Pkg of 50 | Bio-Rad |
1725124 | iTaq™ Universal SYBR® Green Supermix, 2,500 x 20 μl rxns, 25 ml (5 x 5 ml) | ea | Bio-Rad |
1653308 | Mini-PROTEAN® Short Plates | Pkg of 5 | Bio-Rad |
1652088 | Gene Pulser®/MicroPulser™ Electroporation Cuvettes, 0.4 cm gap | Pkg of 50 | Bio-Rad |
1610747 | 4x Laemmli Sample Buffer | 10ml | Bio-Rad |
1658001 | Mini-PROTEAN® Tetra Cell | ea | Bio-Rad |
1620177 | Immun-Blot PVDF Membrane, Roll, 26 cm x 3.3 m #1620177 | 26 cm x 3.3 m | Bio-Rad |
HSP9601 | Hard-Shell® 96-Well PCR Plates, low profile, thin wall, skirted, white/clear | Pkg of 50 | Bio-Rad |
1610732 | TRIS/GLYCINE/SDS 10X BUFR,1 LT | ea | Bio-Rad |
1250140 | AMINEX HPX-87H,300X7.8MM | Pkg of 1, 300 x 7.8 mm | Bio-Rad |
171203060 | Bio-Plex Calibration Kit | KIT | Bio-Rad |
1863024 | ddPCR Supermix for Probes (No dUTP) | 5 ml (5 x 1 ml) | Bio-Rad |
1653311 | Mini-PROTEAN® Spacer Plates with 1.0 mm Integrated Spacers | 5塊/盒 | Bio-Rad |
1703930 | Mini Trans-Blot Electrophoretic Transfer Cell | ea | Bio-Rad |
1658004 | Mini-PROTEAN® Tetra Vertical Electrophoresis Cell for Mini Precast Gels, 4-gel | kit | Bio-Rad |
1645050 | PowerPac™ Basic Power Supply | 臺 | Bio-Rad |
1705061 | Clarity Western ECL Substrate, 500 ml | 500ml | Bio-Rad |
5000205 | Quick Start™ Bradford 1x Dye Reagent #5000205 | 1L | Bio-Rad |
7311550 | Poly-Prep Chromatography Columns | Pkg of 50 | Bio-Rad |
4561086 | 4-15% Mini-PROTEAN TGXTM 預制蛋白質凝膠,15孔,15uL | 10塊/盒 | Bio-Rad |
171000055 | Sheath Fluid | 20L | Bio-Rad |
TCS0803 | 0.2 ml Flat PCR Tube 8-Cap Strips, optical, ultraclear #TCS0803 | Pkg of 120 | Bio-Rad |
5671085 | –15% Criterion™ TGX™ Precast Midi Protein Gel, 26 well, 15 µl | 1個/盒 | Bio-Rad |
1864021 | 一步法RNA數字PCR檢測試劑盒 | kit | Bio-Rad |
1864110 | Automated Droplet Generation Oil for Probes | 140 ml | Bio-Rad |
1863004 | ddPCR™ Droplet Reader Oil | 2 x 1 L | Bio-Rad |
1703935 | Mini Trans-Blot Module #1703935 | ea | Bio-Rad |
1725125 | iTaq™ Universal SYBR® Green Supermix, 5,000 x 20 µl rxns, 50 ml (10 x 5 ml) #1725125 | 5,000 x 20 ul reactions | Bio-Rad |
1658037 | Mini-PROTEAN® Tetra Electrode Assembly #1658037 | Pkg of 1 | Bio-Rad |
1708891 | iScript™ cDNA Synthesis Kit | 100 x 20 ul | Bio-Rad |
1705060 | Clarity Western ECL Substrate, 200ml | 200ml | Bio-Rad |
171203001 | Bio-Plex® Validation Kit 4.0 | kit | Bio-Rad |
10026938 | 轉膜液western blot轉印緩沖液 TBT buffer | 1L | Bio-Rad |
1610611-5g | Dithiothreitol (DTT) | 5g | Bio-Rad |
7321010 | Econo-Pac® Chromatography Columns, Pkg of 50 | Pkg of 50 | Bio-Rad |
369 | Lyphochek Tumor Marker Plus Control. Level 3 | 6 x 2 ml /盒 | Bio-Rad |
MCA1957 | CD68 antibody | FA-11 | 0.25 mg | Bio-Rad |
1652082 | Gene Pulser/MicroPulser Electroporation Cuvettes, 0.2 cm gap #1652082 | Pkg of 5 | Bio-Rad |
4561083 | 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels, 10-well, 30 μ | Pkg of 10 | Bio-Rad |
4561033 | This gel has been replaced with 10% Mini-PROTEAN TGX Precast Protein Gels, 10-well, 30 μl | pkg 10 | Bio-Rad |
12001925 | Droplet Digital PCR Consumables | pkg of 25 | Bio-Rad |
1610737 | 2x Laemmli Sample Buffer #1610737 | 30ml | Bio-Rad |
HCA177 | HUMAN ANTI TRASTUZUMAB | 0.1 mg | Bio-Rad |
1658033 | Mini-PROTEAN® Tetra Cell, Mini Trans-Blot® Mod/ PP BPS | ea | Bio-Rad |
1537302 | Affi-Gel Blue Gel | 100ml | Bio-Rad |
1652676 | Gene Pulser Electroporation Buffer #1652676 | 10 x 1.8 ml | Bio-Rad |
MCA2260 | RAT ANTI RITUXIMAB | 0.2 mg | Bio-Rad |
1610781 | 10% Tween 20, Nonionic Detergent | 1L | Bio-Rad |
1653312 | Mini-PROTEAN® Spacer Plates with 1.5 mm Integrated Spacers | Pkg of 5 | Bio-Rad |
368 | Lyphochek Tumor Marker Plus Control、Level 2 | 6 x 2 ml /盒 | Bio-Rad |
367 | Lyphochek Tumor Marker Plus Control. Level 1 | 6 x 2 ml /盒 | Bio-Rad |
MCA337 | Mouse anti Human FSH Intact | 0.5 mg | Bio-Rad |
1704085 | Thick Blot Filter Paper, Precut, 9.5 x 15.2 cm | Pkg of 50 | Bio-Rad |
1652441 | 15 m, tubing for coating with DNA- or RNA-gold microcarrier complexes, used with the Helios® gene gun, .125" (3.175 mm) OD, .093" (2.36 mm) ID | Tefzel Tubing, 15 m | Bio-Rad |
HSP9655 | Pkg of 50, white shell/white well PCR plate for higher fluorescent signals, rigid 2-component design | Pkg of 50 | Bio-Rad |
HCA282 | HUMAN ANTI DENOSUMAB | 0.1 mg | Bio-Rad |
1421253 | Chelex 100 Chelating Resin, molecular biology grade, 200–400 mesh, sodium form, 50 g | 50g | Bio-Rad |
1706516 | Goat Anti-Mouse IgG (H + L)-HRP Conjugate | 2ml | Bio-Rad |
1704157 | Transfer Pack, Midi, PVDF, 10 Pack | 10 Pack | Bio-Rad |
1401441 | AG® 1-X8 Anion Exchange Resin, analytical grade, 100–200 mesh, chloride form, 500 g #1401441 | 500g | Bio-Rad |
1610183 | TGX Stain-Free™ FastCast™ Acrylamide Kit, 10% #1610183 | Kit | Bio-Rad |
1704158 | Trans-Blot Turbo Mini 0.2 µm Nitrocellulose Transfer Packs #1704158 | Pkg of 10 | Bio-Rad |
HCA204 | HUMAN ANTI ADALIMUMAB | 0.1mg | Bio-Rad |
MCA749PE | Mouse anti Guinea Pig CD4:RPE | 100TESTS | Bio-Rad |
1864108 | DG32 Automated Droplet Generator Cartridges | Pkg of 30 | Bio-Rad |
1706545 | Bio-Dot Apparatus | ea | Bio-Rad |
1814040 | PCR Plate Heat Seal, foil, pierceable | Pkg of 100 | Bio-Rad |
1703932 | Thick Blot Filter Paper, Precut, 7.5 x 10 cm | Pkg of 50 | Bio-Rad |
1610377 | Precision Plus Protein™ Dual Xtra Prestained Protein Standards | 500ul | Bio-Rad |
1620112 | Nitrocellulose Membrane, Roll, 0.2 µm, 30 cm x 3.5 m | Pkg of 1 roll | Bio-Rad |
1421241 | AG® 50W-X2 Cation Exchange Resin, analytical grade, 100–200 mesh, hydrogen form, 500 g | 500g | Bio-Rad |
1631112 | 40% BioLyte® 3/10 Ampholyte | 10ml | Bio-Rad |
1450011 | Cell Counting Slides for TC10™/TC20™ Cell Counter, Dual-Chamber, 30 slides, 60 counts #1450011 | 30 slide | Bio-Rad |
1620115 | Nitrocellulose Membrane, Roll, 0.45 μm, 30 cm x 3.5 m | Pkg of 1 roll | Bio-Rad |
7316214 | Poly-Prep® Columns, AG® 50W-X8, hydrogen form #7316214 | ea | Bio-Rad |
1250095 | AMINEX HPX-87C,300X7.8MM | ea | Bio-Rad |
1864006 | QX200 Droplet Generation Oil for EvaGreen #1864006 | 70 ml (10 x 7 ml) | Bio-Rad |
1653359 | Mini-PROTEAN® Comb, 10-well, 1.0 mm, 44 μl #1653359 | Pkg of 5 | Bio-Rad |
1863052 | ddPCR™ Buffer Control for Probes | 2 x 4.5 ml | Bio-Rad |
1610363 | Precision Plus Protein™ Unstained Protein Standards, Strep-tagged recombinant | 1ml | Bio-Rad |
1706404 | Blotting-Grade Blocker #1706404 | 300g | Bio-Rad |
1863023 | ddPCR™ Supermix for Probes (No dUTP) | 2 ml (2 x 1 ml) | Bio-Rad |
HCA203 | HUMAN ANTI ADALIMUMAB | 0.1 mg | Bio-Rad |
1703940 | Trans-Blot® SD Semi-Dry Electrophoretic Transfer Cell | kit | Bio-Rad |
1725281 | SsoAdvanced Universal Probes Supermix, 500 x 20 µl rxns, 5 ml (5 x 1 ml) #1725281 | 5 x 1 ml | Bio-Rad |
1864008 | DG8™ Cartridges for QX200™/QX100™ Droplet Generator #1864008 | Pkg of 24 | Bio-Rad |
1725284 | SsoAdvanced Universal Probes Supermix | 一盒 | Bio-Rad |
2200101CN | 糖化血紅蛋白A1c檢測試劑盒(HPLC法) | 400T | Bio-Rad |
4561043 | This gel has been replaced with 12% Mini-PROTEAN TGX Precast Protein Gels, 10-well, 30 μl, pkg 10 | pkg 10 | Bio-Rad |
1704273 | Trans-Blot Turbo RTA Midi 0.2 µm PVDF Transfer Kit, for 40 blots | 1kit | Bio-Rad |
1610375 | PREC PLUS STD KALEIDOSCOPE | 500ul | Bio-Rad |
1645070 | PowerPac Universal Power Supply | ea | Bio-Rad |
1658038 | Mini-PROTEAN® Tetra Companion Running Module #1658038 | Pkg of 1 | Bio-Rad |
1703967 | Extra Thick Blot Filter Paper, Precut, 8.6 x 13.5 cm | 60張 | Bio-Rad |
1704017 | Mini-PROTEAN II Multiscreen Apparatus | Pkg of 1 | Bio-Rad |
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